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Full text of "Pathogenic Bacteria"

156

PATHOGENIC BACTERIA.

hydrogen. In using Botkin's apparatus the uncovered
Petri dishes are placed one above the other in the rack c,
and covered with the bell-glass A. Liquid paraffin is
poured in the dish B until it is about half full. From a

Kipp's apparatus hydrogen
gas enters the little rubber
tube #, subsequently escap-
ing by the tube b. When
only pure hydrogen escapes
the rubber tubes a and b are
withdrawn, and the appa-
ratus remains filled with hy-
drogen. Lest a little oxygen
should remain, it is best to
have the dishes at the top
and bottom of the rack filled
with alkaline pyrogallic
acid. Tetanus can be cul-
tivated in this apparatus.

The jars  recently intro-
duced by Novy are similar

FIG. 38.óBotkin's apparatus for mak-
ing anaerobic cultures.

in     principle,     depending
upon the replacement of the air by hydrogen.    They are

FIG. 39.óNovy's jars for anaerobic cultures.

so constructed that when the stopper occupies a certain
relative position to the neck the gas can enter and exit,